Protein Labeling Reagents
Dojindo Molecular Technologies Inc DAPGreen–Autophagy Detection(5 nmol)
DAPGreen is used to detect autophagy in live cells. Autophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. The small fluorescent molecule DAPGreen is used to detect autophagosomes and autolysosomes. The mechanism has been suggested to be that the dye is incorporated into the autophagosome during double-membrane formation via structural features and then emits fluorescence under hydrophobic conditions (λex: 450 nm, λem: 535 nm). DALGreen is cell-permeable, has no requirement of transfection method, and enables live-cell imaging with fluorescence microscopy, plate reader, and flow cytometry. For monitoring only autolysosomes, we recommend DALGreen [D675], as it allows the detection of phagosome-lysosome fusion. Dojindo offers various reagents for autophagy research. (https://www.dojindo.com/autophagy-research/#jmp-b)
Proteintech Group Inc Histone-Label Atto488
Histone-Label* is a fluorescent chromatin probe for direct staining of chromosomes and nuclei. It consists of an anti-histone VHH conjugated to the fluorescent dye ATTO488. For IF, microscopy of histones, chromosomes, and nuclei.
MilliporeSigma TMB/E ULTRA SENSITIVE
TMB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (soluble)
Cayman Chemical PhosphIn-bIotIn 5mg
A labeling reagent that selectively reacts with azido groups on modified proteins through the Staudinger ligation reaction; can be used in common avidin-based biochemical techniques in whole cells or by blotting experiments following SDS-PAGE; has been used successfully in conjunction with Daz-1 or Daz-2 to label and detect sulfenic acid sites in proteins
![eMolecules Broadpharm / Sulfo-Cy7 azide / 1mg / 771351566 / BP-28906 / / / [null] / 829.090 / C40H49KN6O7S2](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502460045.PNG-250.jpg)
eMolecules Broadpharm / Sulfo-Cy7 azide / 1mg / 771351566 / BP-28906 / / / [null] / 829.090 / C40H49KN6O7S2
Broadpharm / Sulfo-Cy7 azide / 1mg / 771351566 / BP-28906 / / / [null] / 829.090 / C40H49KN6O7S2